Figure 1

Multiple sequence alignment of the plastid threonine (trnT-GGU) gene and the position of pseudogenes within the phylogenetic tree. (a) All functional parts of the gene have been noted above the alignment. The insertion occurring in the acceptor stem is highlighted. Co-occurrence of mutational events in some species are shown above and below the alignment. (b) The black block indicates the starting node of the insertion event in the 5′ acceptor stem and pseudogene detected by ARAGORN among species of the ‘Core Asteraceae’ clade, whereas the purple block indicates the presence of pseudogenes based on tRNAscan-SE. Leaves of the phylogenetic tree from Barnadesioideae to Corymbioideae represent subfamilies of Asteraceae, while all other leaves of phylogenetic trees represent 13 tribes of the Asteroideae subfamily (indicated in blue background), followed by the icon size photo of a representative species used in our analysis. The species from 13 tribes of Asteroideae were included in the analysis and their names are noted at each node in the highlighted background. The representative photos of each subfamily and tribe are included in the figure and the species names are provided in front of each photo.