Figure 10

ssDNA binding by RecA variants in the presence of various cofactors. The histogram represents the percentage of fluorescence corresponding to the bound ssDNA compared to the total ssDNA fluorescence. ssDNA binding was assessed in the presence of ATP (white bars with black dots), which is expected to be hydrolysed by RecA and to generate ssDNA-RecA filament dissociation, and in the presence of ATPγS (blue bars), in the presence of which the filament is in a non-dissociating active configuration. The reaction was carried out for 10 min, except for DdRecA in the presence of ATPγS, for which the reaction lasted 20 min because the reaction was observed to be slower (results not shown) (this is indicated by filled triangle in the figure).