Figure 3

3D structure of RecA variant-ssDNA nucleoprotein filaments. Side view of the 3D structure of 3 monomers of an E. coli RecA-ssDNA nucleoprotein filament. Each monomer is coloured violet, red and grey. Amino acids modified in the different variants are represented as spheres and are coloured cyan for the violet monomer, green for the red monomer and pink for the grey monomer. The mutations are indicated by arrows. ssDNA is shown in yellow. (A) The EcPa variant includes the L29M, I102D, I159M + S162A + M164V and L178I + A179T + L182I mutations. (B) The EcRecAV1 variant includes the L29M, V79L and A289S mutations. This mutant is also truncated by 17 amino acids at the C-tail. As the tail is disordered, it does not appear in the 3D structure. (C) The EcRecAV2 variant includes R28D, L29M, V79L and I102L. The figure was generated using the PyMol program¹⁸ and models provided by Prentiss and Prevost¹⁹.