Figure 4

Growth curves of strains producing RecA variants. MG1655 nalR lacZΔP recA::Cm cells transformed with pQE-80L plasmid derivatives containing each of the seven recA variants induced by 1 mM IPTG were cultured at 37 °C in microplate and the growth was analysed by measuring the OD600 with Tecan Spark for 24 h. Triplicates were performed. MG1655 nalR lacZΔP RecA + strain (red dotted curve), ΔrecA strain (red curve), ∆recA strains producing EcRecA (green curve), DdRecA (blue curve), PaRecA (grey curve), DrRecA (black curve), EcPa variant (orange curve), EcRecAV1 variant (yellow curve) or EcRecAV2 variant (violet curve) are shown.