Table 2 Selected H3 sequences for experimental validations.

Design ID	H3 sequence	H3 length^a	H3 sequence^b		K_D (nM)	\({K}_{\text{D}}^{\text{Parent}}/{K}_{\text{D}}^{\text{Design}}\)	\({K}_{\text{D}}^{\text{minH3}}/{K}_{\text{D}}^{\text{Design}}\)	T_m (C)^d
Design ID	H3 sequence	H3 length^a	Identity	Similarity	K_D (nM)			T_m (C)^d
minH3	SRWGMDY	7	100	100	16,000	0.0063	1.0
Parent	SRWGGDGFYAMDY	13	100	100	100	1.0	160	77.5
16_0325	ARGGAVAGTGVYYFDY	16	31	31	8^c	13	2000	81.3
14_0112	AKGGSSSGPYHFEY	14	21	29	8^c	13	2000	81.3
14_0472	ARGIAVAGAYYFDY	14	29	29	59,000	0.0017	0.27	83.4
13_0346	ARGGSFYYYYMDV	13	39	54	80	1.3	200	83.5
16_0102	AKGWEGTTVTLTPVDY	16	13	19	50,000	0.002	0.32	76.5
15_0485	ARHGVRGYYYYYMDV	15	27	40	310	0.32	52	78.8
14_0905	VRGGYLRDYYGMDV	14	21	29	5000	0.02	3.2	81.1
14_0130	AKLGIGYYYYGMDV	14	29	36	2800	0.04	5.7	76.6
14_0822	GRSGPRLGMYYFDF	14	21	29	> 50,000	< 0.002	< 0.3	81.1
12_0327	ARGRKYSSSFDY	12	25	33	1600	0.063	10	79.4
14_0480	ARGLERSGNYYLDY	14	29	36	2720	0.037	5.9	79.7
14_0129	AKLGGQGSYYHFDY	14	29	36	3480	0.029	4.6	75.2
16_0460	ARPSGGSRSWLYYFDY	16	25	25	4500	0.022	3.6	76.3
14_0490	ARGNEAGYYYGMDV	14	21	29	3590	0.028	4.5	79.3
14_0622	ARNGGDSYSGYFQH	14	7.1	14	4970	0.02	3.2	83.3
14_0688	ARSGRDAYNYYFDS	14	14	14	3600	0.028	4.4	86.5

^aLoop sequence length, in amino-acid residues, according to the IGMT definition⁴⁶.
^bH3 sequence identity and similarity relative to the parental H3 sequence as determined using MOE (Chemical Computing Group) alignment tools.
Highlighted in bold are the designs structurally characterized by X-ray crystallography.
^cPoor fit to 1:1 Langmuir binding model.
^dSee DSC thermograms (Figure S2).

Quick links

Search