Figure 6

Effect of periostin receptor silencing on NSCLC cells in vivo. ITB3-depleted A549 or NCI-H520 cells were mixed with or without DIPF in a 5:1 ratio, co-injected subcutaneously into nude mice (n = 5), and sacrificed 28 days after co-injection. (a,b) Mice with ITB3-depleted A549 (NCI-H520) cells with or without DIPF co-injection were sacrificed 28 days after co-injection and tumor weight was measured. (c) Sections were prepared from xenograft tumors of A549 (NCI-H520) stained with anti-Ki-67 antibody. (d,e) Ki-67-positive cells were counted in xenograft tumors of A549 (d) and NCI-H520 (e). Results are shown as mean ± SD expressed as the percentage of positively stained cells compared with the total number of cells. Solid squares show enlarged images and scale bars, 100 μm in (c). *P < 0.05, **P < 0.01. n.s., non-significant; ITB3, integrin β3; DIPF, diseased human lung fibroblasts with IPF.