Figure 6
From: Cardiac expression of microRNA-7 is associated with adverse cardiac remodeling
Validation of proteomic studies, and mitochondrial structure–function ananlysis (a), Cardiac lysates (100 µg) from an independent set of NTg and miRNA-7 Tg mice were immunoblotted with anti-alpha synuclein, anti-VPS26, or anti-PPIF and stripped and reblotted with GAPDH (left panels). Right panel, cumulative data *p < 0.001 vs. NTg (n = 7). (b) Immunoblotting was performed on cardiac lysates (100 µg) using anti-GHITM and the blots were stripped and reblotted with anti-NDUFA9 followed by actin (left panels). Right panel, cumulative data *p < 0.001 vs. NTg (GHITM) & *p < 0.01 vs. NTg (NDUFA9) (n = 4). (c) Mitochondria isolated from miRNA-7 Tg and NTg underwent high resolution oxygen consumption assessment using the Oroboros high resolution respirometry wherein maximum respiration is presented as a measure of mitochondrial function. *p < 0.01 (n = 3). (d) Transmission electron microscopy of the heart sections from NTg and Tg shows marked alteration in mitochondrial ultra-structure. NTg mice are characterized by well-organized mitochondria aligned with sarcomeres. Tg mitochondria are disorganized and rounded in shape reflecting alterations in mitochondrial structural components (n = 5). Upper panel—lower magnification (scale bar—2 μm); Lower panel—higher magnification (scale bar—1 μm).
