Table 1 Cell lines with species identity determined by sequencing that matched institute records or were previously unknown.
From: High-throughput sequencing for species authentication and contamination detection of 63 cell lines
Cell line | Species | cDNA run | Total reads | Number of reads mapped to COX1 | Breadth of coverage of COX1 (%) | Mean depth of coverage of COX1 | Total variants | GenBank reference accession |
|---|---|---|---|---|---|---|---|---|
3T6-Swiss Albino | Mus musculus | 2 | 715,514 | 128 | 99.3 | 12.3 | 0 | KY018919.1 |
A549 | Homo sapiens | 2 | 2,306,896 | 1374 | 100.0 | 141.8 | 0 | MW389273.1 |
BHK-21 | Mesocricetus auratus | 2 | 706,654 | 39 | 99.2 | 3.4 | 0 | EU660218.1 |
CEF | Gallus gallus | 2 | 711,596 | 1882 | 100.0 | 238.5 | 0 | MN013407.1 |
CHCC-OU2 | Gallus gallus | 2 | 2,327,710 | 9681 | 100.0 | 1,070.4 | 0 | MN013407.1 |
CHO-K1 | Cricetulus griseus | 2 | 3,254,926 | 300 | 100.0 | 20.4 | 0 | KX576660.1 |
COS-1 | Cercopithecus aethiops | 2 | 1,438,372 | 141 | 99.5 | 11.0 | 0 | MN816163.1 |
CPAE | Bos taurus | 1 | 5,933,398 | 1676 | 100.0 | 146.6 | 0 | MF663794.1 |
CV-1 | Cercopithecus aethiops | 2 | 923,170 | 277 | 100.0 | 22.3 | 0 | MN816163.1 |
DE | Anas platyrhynchus | 2 | 840,902 | 505 | 100.0 | 51.8 | 0 | MH744426.1 |
DF-1 | Gallus gallus | 2 | 1,145,492 | 805 | 100.0 | 88.6 | 0 | MK163563.1 |
Efk-1B | Eptesicus fuscus | 2 | 1,015,466 | 311 | 95.5 | 25.6 | 2 | MF143474.1 |
Efk-2F | Eptesicus fuscus | 2 | 1,137,244 | 257 | 95.5 | 20.9 | 2 | MF143474.1 |
Efk-3B | Eptesicus fuscus | 2 | 1,051,616 | 246 | 95.4 | 18.8 | 2 | MF143474.1 |
EL4-IL2 | Mus musculus | 2 | 2,016,368 | 735 | 100.0 | 86.3 | 1 | KY018919.1 |
H1299 | Homo sapiens | 2 | 1,322,326 | 392 | 100.0 | 37.2 | 0 | MW389273.1 |
HEK-293 | Homo sapiens | 2 | 2,997,732 | 845 | 100.0 | 95.0 | 0 | X93334.1 |
IB-RS-2 Clone D10 | Sus scrofa | 2 | 4,731,602 | 2846 | 100.0 | 282.8 | 1 | MF183225.1 |
IPAM 3C10 | Sus scrofa | 2 | 6,759,802 | 2827 | 100.0 | 238.2 | 0 | MH603005.1 |
IPAM 3C8 | Sus scrofa | 2 | 1,701,142 | 229 | 100.0 | 17.0 | 0 | MT199606.1 |
IPAM 3E8 | Sus scrofa | 2 | 1,214,488 | 160 | 100.0 | 13.6 | 0 | MG250562.1 |
IPAM 3F6 | Sus scrofa | 2 | 1,948,740 | 434 | 100.0 | 33.5 | 0 | MG250562.1 |
L929 | Mus musculus | 2 | 1,134,052 | 164 | 100.0 | 21.0 | 0 | EU315228.1 |
LK-W(14) | Ovis aries | 2 | 3,834,432 | 5324 | 100.0 | 613.8 | 0 | EF490453.1 |
LLC-PK1 | Sus scrofa | 2 | 862,572 | 423 | 100.0 | 48.2 | 0 | AF486866.1 |
LMH | Gallus gallus | 2 | 847,388 | 524 | 100.0 | 56.5 | 0 | MN013407.1 |
LT | Ovis aries | 2 | 1,165,940 | 452 | 100.0 | 38.0 | 0 | EF490453.1 |
MDBK-HS-1 | Bos taurus | 2 | 2,071,902 | 846 | 100.0 | 98.2 | 0 | MN714195.1 |
MDBK-HS-2 | Bos taurus | 2 | 658,222 | 103 | 100.0 | 10.9 | 0 | MN714195.1 |
MDCK SIAT1 | Canis familiaris | 2 | 1,270,062 | 506 | 100.0 | 53.6 | 0 | KM061581.1 |
MDCK2 | Canis familiaris | 2 | 1,474,730 | 2038 | 100.0 | 236.5 | 0 | KM061555.1 |
MDCK-PGOK | Canis familiaris | 2 | 1,242,568 | 153 | 100.0 | 18.9 | 0 | KM061581.1 |
OA3.Ts | Ovis aries | 2 | 1,148,750 | 647 | 100.0 | 55.0 | 0 | KU681212.1 |
OA4K/s1 | Ovis aries | 2 | 900,506 | 182 | 98.5 | 13.1 | 0 | MT768116.1 |
P3X63-Ag8-653 | Mus musculus | 2 | 1,999,368 | 2896 | 100.0 | 305.4 | 0 | AY533105.1 |
PaKi | Pteropus alecto | 2 | 1,002,720 | 223 | 99.9 | 19.7 | 0 | KF726143.1 |
PK-15 (PCV-) | Sus scrofa | 1 | 1,116,214 | 1123 | 100.0 | 98.1 | 0 | KT279758.1 |
PK-15 (PCV +) | Sus scrofa | 1 | 3,916,490 | 757 | 100.0 | 68.2 | 0 | KT279758.1 |
QT-35 | Coturnix japonica | 2 | 824,636 | 1183 | 100.0 | 133.8 | 0 | KX712089.1 |
RK13 | Oryctolagus cuniculus | 2 | 729,418 | 320 | 100.0 | 34.3 | 5 | MN296708.1 |
SC-1 | Gallus gallus | 2 | 1,296,288 | 1101 | 100.0 | 91.0 | 0 | GU261694.1 |
SIRC | Oryctolagus cuniculus | 2 | 614,400 | 146 | 100.0 | 15.2 | 1 | MN296708.1 |
SK-6 | Sus scofa | 2 | 867,492 | 162 | 100.0 | 18.0 | 0 | MG250562.1 |
ST | Sus scrofa | 1 | 2,051,240 | 707 | 100.0 | 60.5 | 0 | AF486866.1 |
TG180 | Mus musculus | 2 | 4,749,142 | 1263 | 100.0 | 130.8 | 0 | KP260515.1 |
Tni | Trichoplusia ni | 2 | 1,449,444 | 348 | 100.0 | 43.9 | 0 | NC_045936.1 |
WSL-R-HP | Sus scrofa | 2 | 913,228 | 804 | 100.0 | 65.8 | 0 | MF183225.1 |
ZZR | Capra aegregrus hircus | 2 | 988,600 | 262 | 100.0 | 28.0 | 0 | MH229952.1 |
Previously undefined cell lines | ||||||||
BD41/31 | Sus scrofa | 2 | 1,020,194 | 413 | 100.0 | 47.5 | 0 | MH603005.1 |
MARC-145 | Chlorocebus pygerythrus | 2 | 594,988 | 222 | 100.0 | 14.6 | 0 | MT481926.1 |
MRC5 | Homo sapiens | 2 | 858,666 | 249 | 100.0 | 23.7 | 0 | MK059615.1 |
N418 | Mus musculus | 2 | 1,180,212 | 194 | 100.0 | 22.2 | 0 | KY018919.1 |
OUR-1 | Mus musculus | 2 | 1,615,188 | 129 | 100.0 | 10.1 | 0 | KP260516.1 |
