Table 2 Cell lines in which a different species from institution records was identified.

From: High-throughput sequencing for species authentication and contamination detection of 63 cell lines

Cell line

Species

cDNA run

Total reads

Number of reads mapped to COX1/CytB

Breadth of coverage of COX1/CytB (%)

Mean depth of coverage of COX1/CytB

Total variants

Reference mitogenome accession

LFBK-αVβ6

Sus scrofa

2

4,351,208

880

100.0

91.0

0

JN601075.1

Bos taurus

77

44.1

8.2

98

MN200869.1

SCP-HS

Bos taurus

2

511,706

202

100.0

23.7

0

MF663794.1

Ovis aries

85

100.0

11.1

176

KU681212.1

Same genus

BGMK

Chlorocebus pygerythrus

2

896,826

501

100.0

50.4

6

EF597501.1

320

100.0

40.1

5

JX983774.1

Chlorocebus aethiops

484

100.0

48.8

47

MN816163.1

287

100.0

36.8

47

CGBQ

Anser cygnoides

2

2,426,176

10,021

100.0

1,179.6

0

MN356388.1

3363

100.0

536.1

0

MK102803.1

Anser anser

10,010

100.0

1,178.4

9

MN122908.1

3352

99.9

534.7

19

MA-104

Chlorocebus pygerythrus

2

947,256

165

100.0

14.6

0

MT481926.1

127

100.0

13.5

0

Chlorocebus aethiops

149

100.0

13.2

55

MN816163.1

96

100.0

9.7

49

PaLu

Pteropus ornatus

2

1,030,394

197

100.0

17.2

39

NC_046926.1

97

100.0

10.1

41

Pteropus alecto

192

100.0

16.9

49

KF726143.1

96

100.0

10.4

37

PaSPT

Pteropus ornatus

2

936,338

195

100.0

25.7

40

NC_046926.1

93

100.0

12.7

38

Pteropus alecto

194

100.0

25.6

50

KF726143.1

91

100.0

12.4

37

Vero

Chlorocebus sabeus

2

461,114

311

100.0

25.1

3

JQ256913.1

223

100.0

23.3

2

EF597503.1

Chlorocebus aethiops

285

100.0

23.4

67

MN816163.1

161

99.1

17.9

67

Vero Nectin-4

Chlorocebus sabeus

2

633,176

238

100.0

28.0

3

JQ256913.1

294

100.0

40.2

2

EF597503.1

Chlorocebus aethiops

207

100.0

26.2

65

MN816163.1

223

100.0

33.5

70

Vero-76

Chlorocebus sabeus

1

1,284,012

618

100.0

50.3

3

EF597503.1

598

100.0

64.8

2

EF597503.1

Chlorocebus aethiops

583

100.0

47.4

67

MN816163.1

534

100.0

59.5

70

  1. Species names in bold are the observed species while the non-bolded names are the expected species based on laboratory records. Bold values indicate results to COX1 reference while non-bolded cells are to CytB reference. Variants were only called if the read depth had a minimum coverage of 3×. Threshold for coverage depth for calling breadth and depth of coverage was set to 3, while the minimum variant frequency = 0.75.