Figure 2
Expression of the GABAAR β1 subunit in the entorhinal cortex, hippocampal CA1 region and dentate gyrus. (a, b, c) Representative immunoreactive Western Blot bands from younger female (YF), older female (OF), younger male (YM) and older male (OM) tissue homogenates of the ECx, CA1 and DG regions, following incubation with GABAAR β1 antibody. GABAAR β1 and corresponding β-actin band (β1 band size- ~ 53 kDa, β-actin band size ~ 42 kDa) are shown. Each lane was loaded with 20 µg of protein. (d, e, f) Signal intensity graphs for each group comparing GABA β1 Western Blot band was measured and normalised to their corresponding β-actin signal for each age group. The data is graphed as mean ± SEM (N = 4–7). (g, h, i, j, k, l) Correlation graphs for males and females plotting the relationship between age and signal intensity of GABA β1 bands (p * ≤ 0.05).
