Figure 1

Representative images of Immunofluorescence double staining of lymphoma cells. (A) Representative images of HE staining, LAT1 and Ki-67 immunohistochemistry staining in selected cases of aggressive and indolent lymphomas. Aggressive lymphomas (Burkitt lymphoma, Diffuse large B-cell lymphoma (DLBCL), Follicular lymphoma (FL) grade 3B, Peripheral T-cell lymphoma (PTCL)) and indolent lymphomas (Follicular lymphoma (FL) grade 1–2 and grade 2, Small lymphocytic lymphoma (SLL), Marginal zone lymphoma (MZL)). Aggressive lymphomas on the upper row expressed higher levels of LAT1 than indolent lymphomas on the lower row. LAT1 expression levels and patterns were different on the classification of lymphoma aggressiveness. (B) LAT1 staining in the normal lymph node and reactive lymph node as negative controls. Normal lymphocytes did not express LAT1 excluding some activated lymphocytes in the germinal center of the follicle and medulla area. On the other hand, many lymphocytes, especially in the germinal center area, were strongly positive for LAT1 in reactive lymph node tissue. Scale bars indicate 50 µm (zoomed) or 500 µm.