Figure 5 | Scientific Reports

Figure 5

From: Selection of the reference genes for quantitative gene expression by RT-qPCR in the desert plant Stipagrostis pennata

Figure 5

Correlation analysis of the relative expression levels of CL9727 and U3887 between RT-PqCR results and RNA-Seq data. X-axis represented the FPKM value in RNA-Seq data. Y-axis denoted Log2 (the ratio of RT-qPCR) using the method 2−ΔΔCt to calculate the relative expression level. The correlation analysis between RNA-Seq data and RT-qPCR results was applied through detection of the relative expression levels of CL9727 and CL3887 normalized by elF (A, B) and GAPDH (C, D).

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