Table 1 Details of primers and amplification characteristics for RT-qPCR of the eight candidate reference genes.
Gene name | Primer sequence (5′–3′) | Product size (bp) | Ea (%) | R2 |
|---|---|---|---|---|
GAPDH-F | GCGTCAACGAGGACAAGTAC | 151 | 90.9 | 0.994 |
GAPDH-R | GTGGCAGTGATGGAATGAAC | |||
ALDH-F | AACGGCATCCTCTGGG | 127 | 95.3 | 0.995 |
ALDH-R | CCTTCACGGCTTGGTCA | |||
elF-F | CCATCCCTATGAGCCA | 131 | 93.2 | 0.992 |
elF-R | ACTACTGCCAGCCTGAAGACA | |||
ARP6-F | TTCCAAGAAATGGCTCGTT | 117 | 92.1 | 0.989 |
ARP6-R | TCCCATACCTCCCTCTGC | |||
TIP41-F | GGCTCAGGGTTGATGGTG | 126 | 90.8 | 0.996 |
TIP41-R | TGGCAAATGTCGCTTCC | |||
α-TUB-F | TCCAGCGGCAACCTTAG | 180 | 93.4 | 0.998 |
α-TUB-R | TCTCCTTCCTCCATACCTTCT | |||
PP2A-F | TGGAAATAAACAGCCAGAGC | 150 | 95.4 | 0.988 |
PP2A-R | TCAATAAGTCGGATAGAACCCT | |||
HIS-3-F | CGTCGCTACCAGAAGTCG | 119 | 92.1 | 0.999 |
HIS-3-R | GCACCGATGGCAGAAGA |
