Figure 5
From: Nematode CDC-37 and DNJ-13 form complexes and can interact with HSP-90
Mass spectrometry crosslink analysis. (a) Samples used for MS-analysis and crosslinked with DSSG. 1 = CDC-37 monomer, 2 = DNJ-13 monomer, 3 = DNJ-13 dimer, 4 = CDC37·DNJ13 complex made out of one CDC-37 and two DNJ-13 molecules, 5 = complex consisting of two CDC37·DNJ13 complexes. (b) Samples used for MS-analysis, crosslinked with BS3, with the same naming scheme as in (a). (c) Visualization of the crosslinked pairs, obtained from MS-analysis (Table 1). Green lines indicate pairs crosslinked with DSSG; Yellow lines indicate pairs crosslinked with BS3; Red lines indicate the pair crosslinked by both DSSG and BS3.
