Figure 2
From: SuperSelective primer pairs for sensitive detection of rare somatic mutations
Demonstration of the selectivity of PCR assays that utilize a pair of SuperSelective primers for the amplification of rare mutant DNA fragments in the presence of abundant DNA fragments from the entire human genome. All of these reactions contained DNA fragments from 10,000 normal human genomes, including 10,000 copies of the wild-type KRAS sequence. In addition, these reactions contained different quantities of a linearized plasmid containing the KRAS G12C mutant target sequence: either 1,000 copies (shown in green), 100 copies (shown in purple), 10 copies (shown in blue), or 0 copies (shown in red). Each reaction was repeated eight times. The top panel shows the results that were obtained when only one of the primers for the KRAS mutant target was SuperSelective; and the bottom panel shows the results that were obtained when both primers for the KRAS mutant target were SuperSelective. When only one of the two primers was SuperSelective, false-positive signals arose when no KRAS mutant targets were present in the sample. When both primers were SuperSelective, the false-positive signals were suppressed.
