Figure 4 | Scientific Reports

Figure 4

From: An open source extrusion bioprinter based on the E3D motion system and tool changer to enable FRESH and multimaterial bioprinting

Figure 4

Multimaterial bioprinting using the open source bioprinter. (a) Example of fluorescein diacetate (live cells; green) and propidium iodide (dead cells; red) staining of MDA-MB-231 breast cancer cells extruded in laminin bioink directly after extrusion through an 18G needle and following 1 week of culture. (b) Comparative analysis of cell viability following overnight culture (O/N) and one week after extrusion of cells in laminin bioink through an 18 G needle or manually deposited directly from a syringe. (c) Design of the multimaterial construct to be printed using both collagen (green) and cell-laden laminin (red) bioinks, as viewed from above illustrating the print path. (d) Image of the bioprinted multimaterial construct. (e) Col-F staining of the collagen bioink (green) and Celltracker red staining of MDA-MB-231 cells distributed in the laminin bioink.

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