Figure 5

Schisanhenol decreases ASK1, cleaves Caspase-3 and PARP expression, inhibits cytochrome C release and P38 phosphorylation, and increases Bcl-2/Bax expression in SH-SY5Y cells treated with MPP⁺. Cells were treated with (500 μΜ) MPP⁺ for 48 h in the presence of Sal (1 µM, 10 µM, and 50 µM) for 24 h, and the protein levels were examined by Western blot from six separate experiments. Representative blot of ASK1 and Cytochrome C (A); P-P38 and P38 (C); Caspase-3 and PARP (E); Bcl-2 and Bax (G). The density of the blots was quantified, ASK1 and Cytochrome C (B); the ratio of P-P38/P38 (D); Caspase-3 and PARP (F); Bcl-2/Bax ratio (H). Data were expressed as mean ± SD. **P < 0.01, ***P < 0.001 vs corresponding Control group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs corresponding MPP⁺ group; ^&P < 0.05 vs Sal 50 μM + MPP⁺ group. Target Protein (ASK1, Cytochrome C, etc.) levels were quantified using Gel-Pro Analyzer 4.0 software and expressed relative to GAPDH protein levels.