Table 2 Effects of supplemention of 200 mM of choline chloride and choline bicarbonate on incubation liquid metabolites as measured by proton nuclear magnetic resonance (main experiment).

From: The rumen microbiome inhibits methane formation through dietary choline supplementation

Metabolite (mM)

Inoculum

Control

Choline chloride

Choline bicarbonate

SEM

P value

Choline

0.07a

0.06a

18.44b

5.45a

6.836

 < 0.001

Monomethylamine

0.113

0.035

0.116

0.037

0.0572

0.220

Dimethylamine

0.025ab

0.004a

0.105b

0.030ab

0.0359

0.0234

Trimethylamine

0.2a

0.1a

129.8b

134.7b

5.34

 < 0.001

Ethanol

0.41a

0.34a

51.92b

65.51c

5.514

 < 0.001

Acetaldehyde

0

0.008

0.192

0.287

0.2483

0.351

Glycerol

5.40

5.71

7.52

9.17

3.711

0.813

Lactate

0.136

0.133

0.271

1.320

0.8313

0.185

Succinate

0.066a

0.042a

0.043a

1.531b

0.2649

 < 0.001

Formate

0.038a

0.036a

0.125a

2.491b

0.5442

 < 0.001

Methanol

0.021a

0.016a

1.392b

0.676ab

0.3149

 < 0.001

Phenylpropionate

0.565a

0.317b

0.200b

0.195b

0.0384

 < 0.001

2-Methyl-butyrate

0.689a

3.132b

1.060a

0.833a

0.6264

0.0012

  1. All groups other than inoculum (i.e., day 0) are day 15 measurements; mean values with highest standard error of the means (SEM); n = 4.
  2. a–cMean values within a row without common superscripts are significantly different (P < 0.05).