Table 1 Experimental data of macroindentation tests on the 6 PAA hydrogels. D is measured for one indentation picture. ENH-fhd is calculated for a minimum of 10 measurements made on the same gel and ΔE represents the standard deviation.

From: Simple yet effective methods to probe hydrogel stiffness for mechanobiology

Hydrogel

*A/BA%wt

Indenter

δ/h min

δ/h max

ENH-fdh (kPa)

ΔENH-fdh (kPa)

PAA1

4/0.03

SP

0.195

0.227

0.14

0.01

PAA2

4/0.06

SS

0.240

0.274

1.30

0.09

PAA3

4/0.1

SS

0.157

0.194

2.69

0.24

PAA4

5/0.15

SS

0.073

0.089

9.58

0.88

PAA5

5/0.225

SI

0.314

0.364

11.86

0.97

PAA6

8/0.48

SI

0.115

0.150

56.37

8.15

  1. *In this column the wt% of the two monomers are reported. A represent the monofunctional acrylates Acrylamide and BA the bifunctional Bis-acrylamide. For PAA-OH gels, A represent the sum of the monofunctional acrylates Acrylamide and N-hydroxyethyl acrylamide (HEA). The amount of HEA is fixed at 1 mM. Sphere radius (R) and indentation force (F) are reported in Table SI.
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