Figure 9 | Scientific Reports

Figure 9

From: Improving motor neuron-like cell differentiation of hEnSCs by the combination of epothilone B loaded PCL microspheres in optimized 3D collagen hydrogel

Figure 9

Neuronal morphology and neurite outgrowth. (a) Immunofluorescence images indicate neuronal morphology and neurite elongation of MN-like differentiated hEnSCs after 15 days of induction in collagen hydrogel with Mic EpoB (2-Col/PA-CH/Mic EpoB) group. (b) Neurite elongation is shown with red arrows. To display morphology, cells were stained using primary anti-beta III tubulin antibody and secondary Alexa Fluor 488 antibody (× 20 and × 40; Scale bar 100 μm). Neurite length of MN-like cells in 2-Col/PA-CH/Mic EpoB hydrogel compared to TCP and 2-Col/PA-CH differentiation groups at day 15 after differentiation (***P < 0.001).

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