Table 4 Primary and secondary antibodies were used for immunofluorescence staining.

From: Improving motor neuron-like cell differentiation of hEnSCs by the combination of epothilone B loaded PCL microspheres in optimized 3D collagen hydrogel

Antibody/stain

Catalog number

Dilution

Primary antibody

Anti-beta-III-tubulin antibody (Tuj-1) (rabbit monoclonal)

Abcam, ab68193

1:200

Anti-Islet-1 (mouse monoclonal)

Abcam, ab86472

1:200

Anti-choline acetyltransferase (CHAT; mouse monoclonal)

Santa Cruz, sc-55557

1:200

Anti-nestin (NES; mouse monoclonal)

PadzaCo, MN105

1:200

Anti-Homeobox 9 (HB9; mouse monoclonal)

Santa Cruz, sc-515769

1:50

Secondary antibody

Goat anti-rabbit IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 488

Gibco, A-11008

1:500

Goat anti-mouse IgG (H + L) highly cross-adsorbed secondary antibody Alexa Fluor 594

Gibco, A-11032

1:700

Nuclear stain

4′,6-Diamidino-2-phenylindole (DAPI)

Sigma-Aldrich, D8417

1:1000

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