Figure 3

AOFE induces apoptosis through decreased expression of IGFBP-2. (A) FaDu cells were treated with indicated concentration of AOFE for 24 h and then stained with annexin V (green) and PI (red). Images of annexin V/PI-stained cells were taken using an Olympus IX71 fluorescence microscope (magnification LCAch N 10X). (B) FaDu cells were treated with indicated concentration of AOFE for 24 h. The cell lysates were prepared, and analyzed by western blot analysis using anti-IGFBP-2, anti-phosphor mTOR, anti-mTOR and β-actin antibodies.