Figure 3

Effects of Cur-AgNPs on cell viability, cell proliferation, and collagen production. (a) Human dermal fibroblast cells were treated with 0.0016 − 1.60 nM of Cur-AgNPs for 24 h in cytotoxicity testing and in the absence of Cur-AgNPs as a control. (b) Cell proliferation; cells were treated with 0.03 and 0.06 nM of Cur-AgNP and 50 µg/mL of ascorbic acid (Vitamin C) for 7 and 14 days in the measurement of cell production. (c, d) White light transmission microscopy images of human dermal fibroblast cells treated with 0.03 and 0.06 nM of Cur-AgNPs and 50 µg/mL of ascorbic acid (Vitamin C) for 7 and 14 days. Collagens are stained red by Picrosirius dye and photographed at 200 × magnification. (e) Analyzed percentage of collagen production relative to controls. Data were expressed as mean ± SD (n = 3). *P < 0.05 and ***P < 0.001 compared to control within 7 days post-treatment. ^†P < 0.005 and ^†††P < 0.001 compared to control within 14 days post-treatment.