Table 1 Primers used in qPCR.
From: Enhanced wound healing properties of guar gum/curcumin-stabilized silver nanoparticle hydrogels
Gene | Primer sequence | Product (bp) | Melting temperature (°C) | Access number |
|---|---|---|---|---|
β-actin (Actb) | F: 5′-CCCTGGCTCCTAGCACCAT-3′ R: 5′-GATAGAGCCACCAATCCACACA-3′ | 80 | 60 | NM031144 |
Collagen 1 (Col1a1) | F: 5′-CATGTTCAGCTTTGTGGACCT-3′ R: 5′-GCAGCTGACTTCAGGGATGT-3′ | 94 | 60 | NM053304 |
Collagen 3 (Col3a1) | F: 5′-GGGATCCAATGAGGGAGAAT-3′ R: 5′-CCTTGCGTGTTTGATATT-3′ | 128 | 60 | NM032085 |
EGF (Egf) | F: 5′-CTCAGGCCTCTGACTCCGAA-3′ R: 5′-ATGCCGACGAGTCTGAGTTG-3′ | 93 | 60 | NM012842 |
FGF-2 (Fgf2) | F: 5′-GATCCCAAGCGGCTCTACTG-3′ R: 5′-TAGTTTGACGTGTGGGTCGC-3′ | 105 | 60 | NM019305 |
TGF-β1 (Tfgb1) | F: 5′-GGGCTACCATGCCAACTTCTG-3′ R: 5′-GAGGGCAAGGACCTTGCTGTA-3′ | 82 | 60 | NM021578 |
IL-6 (Il6) | F: 5′AACCTGAACCTTCCAAAGATGG-3′ R: 5′-TCTGGCTTGTTCCTCACTACT-3′ | 168 | 55 | NM012589 |
