Figure 2

Expression analysis of Nanobody-R9 (a) and Nanobody-K (b) in E. coli. The figures show SDS-PAGE analysis (Left panels) using Coomassie Brilliant Blue (CBB) detection and western blot analysis (Right panels) using His-probe HRP detection of culture supernatant (Sup), intracellular soluble fraction (S), and intracellular insoluble fraction (I) under the induction at 28 °C for 4–16 h. M show Low molecular weight marker. The arrows indicate the deduced molecular weights of the target proteins. Complete gels and western blots are presented in Supplementary Fig. 1.