Figure 3 | Scientific Reports

Figure 3

From: Enzymatic ligation of an antibody and arginine 9 peptide for efficient and cell-specific siRNA delivery

Figure 3

Enzymatic ligation of Nanobody and R9 by MTG. (a) SDS-PAGE by CBB staining; (b) western blot analysis using His-probe HRP; and (c) western blot analysis using anti-HA antibody. A total of 5–35 µM Q-R9-HA (1–10 times Nanobody concentration) was mixed with 5 µM Nanobody-K and 0.03 U mL−1 MTG and incubated at 20 °C for 6 h. The arrows indicate the deduced molecular weight of each protein. M indicate Low molecular weight marker for protein.

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