Figure 3 | Scientific Reports

Figure 3

From: Genome sequencing of the multicellular alga Astrephomene provides insights into convergent evolution of germ-soma differentiation

Figure 3

Cell-type specific RNA-seq analysis in Astrephomene gubernaculifera. (a) The workflow of isolation of somatic cells and reproductive cells. Mature vegetative colonies in synchronous culture have large reproductive cells and small somatic cells (orange dotted circles). By homogenization and density centrifugation of vegetative colonies, somatic cell and reproductive cells were separated and then subjected to RNA extraction for RNA-seq. Scale bars = 20 μm. (b) The representative enriched terms in somatic and reproductive genes by GO enrichment analysis. Bold texts indicate the GO terms also enriched in somatic and gonidial genes in Volvox carteri32. All other enriched GO terms were listed in Supplementary Table 3. (c) Gene expression ratio of photosynthesis-related genes (Supplementary Data 5). The number in brackets indicates the number of genes in each category. (d) Gene expression ratio of the major carbon metabolic pathways (Supplementary Data 6), nucleotide sugar metabolism and glycosyltransferases (Supplementary Data 7). The number in brackets indicates the number of genes in each category.

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