Table 3 PCR protocol used for amplification of foraminiferal COI fragments.
From: First report of mitochondrial COI in foraminifera and implications for DNA barcoding
Step | Temperature (°C) | Time | Cycles |
|---|---|---|---|
Initial denaturation | 96 | 3 min | |
Denaturation | 96 | 15 s | 40 |
Annealing | 50 | 30 s | |
Extension | 72 | 40 s | |
Final extension | 72 | 5 min |
