Table 2 Expression stability ranking of eleven candidate genes in Apera spica-venti plants resistant and susceptible to pyroxsulam.
Analysis | Sample data | Genes expression stability | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Most stable | Less stable | |||||||||||
GeNorm (M value) | All samples | TBP | ARF1 | GAPC | ACT | CYP28 | UBC | SPS | RCA | RPL23A | UBQ | EF1A |
0.044 | 0.044 | 0.044 | 0.046 | 0.050 | 0.053 | 0.056 | 0.059 | 0.070 | 0.076 | 0.087 | ||
Susceptible | CYP28 | SPS | TBP | ACT | GAPC | ARF1 | UBC | RCA | RPL23A | UBQ | EF1A | |
0.034 | 0.034 | 0.042 | 0.044 | 0.046 | 0.049 | 0.052 | 0.056 | 0.070 | 0.078 | 0.086 | ||
Resistant | TBP | ARF1 | ACT | GAPC | CYP28 | UBC | SPS | RCA | UBQ | RPL23A | EF1A | |
0.034 | 0.034 | 0.034 | 0.035 | 0.038 | 0.041 | 0.045 | 0.048 | 0.053 | 0.058 | 0.068 | ||
BestKeeper (SD) | All samples | TBP | ACT | GAPC | ARF1 | CYP28 | RCA | UBC | SPS | RPL23A | UBQ | EF1A |
0.705 | 0.795 | 0.801 | 0.869 | 0.894 | 0.926 | 0.955 | 1.220 | 1.338 | 1.409 | 1.918 | ||
Susceptible | TBP | ACT | SPS | GAPC | RCA | CYP28 | ARF1 | UBC | RPL23A | UBQ | EF1A | |
0.694 | 0.716 | 0.847 | 0.878 | 0.907 | 0.926 | 0.982 | 1.063 | 1.617 | 1.653 | 1.874 | ||
Resistant | GAPC | ACT | TBP | ARF1 | CYP28 | RCA | UBC | UBQ | SPS | RPL23A | EF1A | |
0.690 | 0.698 | 0.716 | 0.736 | 0.777 | 0.790 | 0.870 | 1.038 | 1.049 | 1.062 | 1.526 | ||
NormFinder (SD) | All samples | CYP28 | TBP | GAPC | ARF1 | RCA | ACT | SPS | UBC | RPL23A | UBQ | EF1A |
0.530 | 0.578 | 0.617 | 0.650 | 0.899 | 0.936 | 0.996 | 1.015 | 1.120 | 1.219 | 1.775 | ||
Susceptible | CYP28 | SPS | GAPC | TBP | ARF1 | ACT | RCA | UBQ | RPL23A | UBC | EF1A | |
0.438 | 0.558 | 0.682 | 0.685 | 0.745 | 0.788 | 0.967 | 1.250 | 1.252 | 1.255 | 1.625 | ||
Resistant | GAPC | TBP | CYP28 | ARF1 | ACT | UBQ | UBC | RCA | RPL23A | SPS | EF1A | |
0.350 | 0.402 | 0.529 | 0.555 | 0.684 | 0.696 | 0.740 | 0.753 | 0.940 | 0.948 | 1.305 | ||
ΔCT (mean SD) | All samples | CYP28 | TBP | GAPC | UBC | ACT | SPS | UBQ | ARF1 | RPL23A | EF1A | RCA |
1.683 | 1.711 | 1.878 | 1.896 | 1.910 | 1.991 | 2.056 | 2.079 | 2.094 | 2.439 | 3.044 | ||
Susceptible | TBP | CYP28 | ACT | UBC | GAPC | EF1A | ARF1 | SPS | RPL23A | UBQ | RCA | |
1.460 | 1.467 | 1.500 | 1.670 | 1.700 | 1.772 | 1.800 | 1.804 | 1.840 | 1.865 | 2.622 | ||
Resistant | CYP28 | TBP | GAPC | SPS | UBC | UBQ | ACT | RPL23A | ARF1 | EF1A | RCA | |
1.388 | 1.458 | 1.516 | 1.525 | 1.529 | 1.545 | 1.700 | 1.723 | 1.814 | 2.217 | 2.813 | ||
RefFinder (Geomean) | All samples | TBP | GAPC | CYP28 | ARF1 | ACT | RCA | UBC | SPS | RPL23A | UBQ | EF1A |
1.190 | 2.280 | 2.510 | 3.720 | 4.740 | 5.230 | 6.960 | 7.740 | 9.000 | 10.000 | 11.000 | ||
Susceptible | CYP28 | SPS | TBP | GAPC | ACT | ARF1 | RCA | UBC | UBQ | RPL23A | EF1A | |
1.570 | 1.860 | 2.830 | 3.220 | 4.160 | 6.190 | 6.190 | 8.460 | 8.970 | 9.490 | 11.000 | ||
Resistant | GAPC | TBP | ARF1 | ACT | CYP28 | UBC | RCA | UBQ | SPS | RPL23A | EF1A | |
1.000 | 2.450 | 2.830 | 3.760 | 3.870 | 6.480 | 6.960 | 7.670 | 8.970 | 9.740 | 11.000 | ||
