Table 1 Sensitivity of assays per each analyte examined.

	LLOD median	LLOD range	LLOQ	ULOQ	Inter-assay CV
	pg/mL	pg/mL	pg/mL	pg/mL	%
Proinflammatory panel
IFNγ	0.37	0.21–0.62	1.76	938	8.16
IL-1β	0.05	0.01–0.07	0.646	375	7.95
IL-4	0.02	0.01–0.03	0.218	158	6.23
IL-6	0.06	0.05–0.09	0.633	488	8.62
IL-8	0.07	0.03–0.14	0.591	375	7.8
TNFα	0.04	0.01–0.13	0.690	248	6.74
Cytokine panel
GMCSF	0.16	0.08–0.19	0.842	750	10.78
IL-17A	0.31	0.19–0.55	3.19	3650	11.45

	fg/mL	fg/mL	fg/mL	fg/mL	%
Ultrasensitive IL-17A
IL-17A	13	N/A	60	140,000	8.67

In this table LLOD, LLOQ, ULOQ for each analyte tested using the MSD proinflammatory panel 1, cytokine panel 1, and MSD S-plex Human IL-17A kits. The units of measurement used in the multiplex assays are pg/ml (10⁻¹² g (picograms) per millilitre), while the units in the ultrasensitive assay are fg/ml (10⁻¹⁵ g (femtograms) per millilitre). The quantitative range of the assay lies between the LLOQ and ULOQ. Inter-assay CV is a measure of the variance between runs of sample replicates on different plates and assesses plate-to-plate consistency—inter-assay CV values < 15% were deemed acceptible⁴². All inter-assay CVs were within the permissible range, indicating a low level of plate-to-plate variability.

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