Figure 7
Detection of the outer membrane fraction in the culture supernatants of exbB2/tolQ and exbD3/tolR mutants. Cells of the wild type (WT), ∆exbB2/tolQ (∆Q), and ∆exbD3/tolR (∆R) were grown in LB with or without 5 mM FA or PCA. As shown in Fig. S13, after centrifugation, the supernatants of the cultures were collected (supernatant), and the cell extracts were prepared. The supernatants were further ultracentrifuged, and the resulting supernatants were collected (ultrasupernatant). Western blot analysis using anti-DdvT and anti-TonB1 antibodies was performed against cell extracts (10 µg of protein), 20 µl of the supernatant, and 20 µl of the ultrasupernatant. The uncropped blot images are shown in Fig. S16. This figure was generated using Canvas X Draw version 7.0.2. (https://www.canvasgfx.com/products/canvas-x-draw).
