Figure 4
From: β-elimination of hyaluronate by red king crab hyaluronidase
Zymogram in polyacrylamide gel containing HA as a substrate: (a)—HPC homogenate (1—protein marker, 2—homogenate after heating in the loading buffer, 3—homogenate after heating in the loading buffer without DTT, 4—homogenate in buffer with DTT without warming up), (b)—S. hyalurolyticus lyase (1—protein marker, 2—after warming up in buffer without DTT, 3—after warming up in buffer with DTT, 4—hyaluronidase in buffer with DTT without warming up). Protein marker PageRuler Prestained Protein Ladder: 180, 130, 100, 70, 55, 40, 35, 25, 15, 10 kDa. The samples were being heated in the loading buffer for 5 min at 95 \({}^\circ \)C.
