Table 1 Some known hyaluronidases.
From: β-elimination of hyaluronate by red king crab hyaluronidase
Hyaluronidase | Opt. \(^\circ \)C and pH | Substrate specificity | Additional information |
|---|---|---|---|
Testicular | ?, 5.021 | HA, chondroitin sulfate22 | Hydrolysis at the \( \beta \)-1,4-glycosidic bond. Final product is tetrasaccharide23. Capable of transglycosylation21,24 |
Leeches | 45 and 6.525 | HA, does not hydrolyze chondroitin sulfate A and C26, and also does not hydrolyze chitin and heparin25 | Hydrolysis at the \( \beta \)-1,3-glycosidic bond, the final product—hexa- and tetrasaccharide—contains glucuronic acid at the reduced end25,26,27 |
Streptomyces hyalurolyticus | 60 and 5.023 | HA, does not hydrolyze chondroitin sulfate, and also does not hydrolyze chitin, heparin, and keratan sulfate23 | The reaction of \(\beta \)-elimination at the \(\beta \)-1,4-glycosidic bond, hexa- and tetrasaccharide are the final products of the endolytic activity of hydrolysis23,28,29 |
Bacillus sp. A5030 | 44 and 6.5 | HA, chondroitin sulfate A, C, D | The reaction of \(\beta \)-elimination at the \(\beta \)-1,4-glycosidic bond, the final product is a dimer |
Cryptococcus laurentii7 (yeast) | 37 and 6.0 | HA, ? | The reaction of \( \beta \)-elimination at the \( \beta \)-1,4-glycosidic bond, the final product is a dimer |
Fistulina hepatica8 (filamentous fungi) | 20 and 4.0 | HA, ? | The reaction of \( \beta \)-elimination at the \( \beta \)-1,4-glycosidic bond, the final product is tetrasaccharide |
