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Figure 2

From: Neural correlates of vocal initiation in the VTA/SNc of juvenile male zebra finches

Figure 2

Histological and electrophysiological confirmation of VTA/SNc single units. (a, b) Histological verification of the recording sites. (a) left, Low magnification of a zebra finch sagittal brain section stained with an antibody against tyrosine hydroxylase (TH). A: anterior, P: posterior. Arrows indicate an electrode track. Right, Magnified view of the area enclosed by the white square in the left panel. A white line indicates an electrode track that entered the TH-positive cell population. The TH-positive cell population is surrounded by a white dotted line which corresponds to the ‘lateral’ brain section in (b). (b) Recording sites from all birds (n = 6) are shown in the example sagittal sections. Each color corresponds to the neuron types (Red: Type-1, Blue: Type-2, Magenta: Type-3, Green: Type-4). Recording sites where singing-related units were obtained are shown. (c) Relationship between spontaneous firing rate and spike half-width. Each circle represents data from a single neuron. Red: Type-1, Blue: Type-2, Magenta: Type-3, Green: Type-4, Gray: No correlation.

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