Table 1 Primer sequences used for quantitative reverse transcriptase polymerase chain reaction.
From: Fibroblast transition to an endothelial “trans” state improves cell reprogramming efficiency
Gene (rat) | Forward | Reverse |
|---|---|---|
cTnT | AGGCTCACTTCGAGAACAGG | ATTGCGAATACGCTGCTGT |
Actc1 | GATTATTGCTCCCCCTGAGCG | GTGTAAGGTAGCCGCCTCAGAA |
Pln | GTGACGATCACAGAAGCCAAGG | TGACAGCAGGCAGCCAAACG |
Gja1 | GAACAGTCTGCCTTTCGCTG | AAGGACCCAGAAGCGCACGT |
CD31 | CTCAGTCGGCTGACAAGATG | AGGCTTGCATAGAGCAGCAT |
CXCL12 | CCCTGCCGATTCTTTGAG | GCTTTTCAGCCTTGCAACA |
ESM1 | GGGGAAACCTGCTACCGTA | CTCCTTGCAATCCATCCCGAAC |
Gata4 | CCGGGCTGTCATCTCACTAT | GAGAGCTTCAGAGCCGACAG |
Mef2c | GACAAGTACAGGAAAATTAACGAAGA | TGGGAGGTGGAACAGCAC |
Tbx5 | GCACAGAAATGATCATCACCAA | GGCCAGTCACCTTCACTTTG |
Twist | AGCTACGCCTTCTCCGTCT | TCCTTCTCTGGAAACAATGACA |
Zeb1 | GCCAACAGACCAGACAGTGTT | CGCATTCGTCATCTTTTACG |
CDH1 | GGCTTGGATTTTGAGGCCAAGC | GCGATCTCCAGACCCACACC |
CDH2 | GGAAGCTGGCATCTATGAAG | CTCCATTGGAGTCACATTGGC |
GAPDH | GGCACAGTCAAGGCTGAGAATG | ATGGTGGTGAAGACGCCAGTA |
ETV2* | AGGGAACAAGCTGGCAGGGCTTGAA | TCCAGCATGTCTCTGCTGTCGCTGT |
