Figure 1
Decreased novelty-induced exploration and increased avoidant behavior in the first 10 and full 60 min of open field following chronic postnatal fluoxetine exposure in C57 mice. (A) Timeline of experiment: The window for developmental administration of fluoxetine and future phases of the experiment are illustrated in faded light grey; Black and grey stripes indicate when baseline adult phenotyping using the OF and FST occurred. (B) Ambulatory movements in the first 10 min of the open field (left panel, unpaired, two-tailed, t-test, p = 0.2846). Same for total 60 min (right panel, unpaired, two-tailed, t-test, p = 0.0712). (C) Rearing movements in the first 10 min (left, unpaired two-tailed, t-test, p = 0.2649). Same for total 60 min (right, unpaired, two-tailed, t-test, **p = 0.0054). (D) Time spent in center in the first 10 min (left panel, two-way ANOVA, F(1, 66) = 8.374, **p = 0.0052 for interaction of sex and treatment, F(1, 66) = 3.739, p = 0.0574 for main effect of sex, F(1, 66) = 5.812, *p = 0.0187 for main effect of treatment; Bonferroni post-hoc for males: **p = 0.0012, n = 14 PNVEH, 18 PNFLX; for females: p > 0.99, n = 17 PNVEH, 21 PNFLX). Same for total 60 min (right panel, unpaired, two-tailed, t-test, p = 0.1813). Light gray squares represent PNVEH animals, n = 31. Black circles and grey stripes represent PNFLX animals, n = 39. Interaction effects from the 2-way ANOVA are represented as significance lines between males and females, and the Bonferroni post-hoc results are depicted as significance lines within the males. Bars represent mean. Error bars represent standard error. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
