Figure 3
From: The von Willebrand factor stamps plasmatic extracellular vesicles from glioblastoma patients
Proteomic analysis of circulating EVs from GBM patients unveils specific cargos. (A) Plasmatic extracellular vesicles (EVs) were separated from six plasmas (3 healthy donors and 3 GBM patients), as described in Fig. 1A. Protein cargoes were quantitatively analyzed through label-free liquid chromatography tandem mass spectrometry (LC–MS/MS). (B) Representation of the top 100 EV-enriched proteins estimated with Exocarta and EVpedia databases, when combining proteomic analysis from 6 samples. (C) DAVID analysis of the top Gene Ontology (GO) functions from the 6 analyzed samples. (D) Principal component analysis of healthy donors and GBM patient samples (left panel). Venn diagram of the detected proteins and their repartition between healthy donor and GBM patient groups (middle and right panel). (E) Heatmap analysis of exclusively expressed proteins. (F, G) Volcano plot and heatmap analysis of differentially expressed proteins.
