Figure 5
From: Endogenous regulation of the Akt pathway by the aryl hydrocarbon receptor (AhR) in lung fibroblasts
Expression of PTEN and p85α protein in Ahr+/+ and Ahr−/− MLFs. (A, C) Ahr+/+ and Ahr−/− MLF were serum-starved for 18 h and cellular protein was collected for Western blot analysis of (A) PTEN and (C) p85α expression. Depicted blots are representative of four independent experiments. Densitometric values were normalized to tubulin and are expressed as fold change relative to that of the Ahr+/+ groups. Results are expressed as mean ± SEM (*p < 0.05). (B) Serum-starved Ahr+/+ and Ahr−/− MLF were treated with 2 mM GSH-MEE or the vehicle control (H2O) for 1 h and cellular protein was collected for Western blot analysis of Akt phosphorylation. Depicted blots are representative of three independent experiments. (D) Ahr+/+ MLFs were transfected with non-targeting control siRNA or siRNA against p85α. Transfected cells were serum-starved for 18 h and cellular protein was collected for western blot analysis of Akt phosphorylation. Representative western blot is shown.
