Figure 6
From: Endogenous regulation of the Akt pathway by the aryl hydrocarbon receptor (AhR) in lung fibroblasts
Effect of LY294002 the Akt pathway on Ahr+/+ and Ahr−/− MLFs. (A, B) Ahr+/+ and Ahr−/− MLFs were treated with either 50 μM LY294002 or the vehicle control (DMSO) for 2 h and cellular protein was collected for Western blot analysis of (A) Akt and (B) GSK-3β phosphorylation. Depicted blots are representative of three independent experiments. Results are expressed as mean ± SEM. (C) Serum-starved Ahr+/+ and Ahr−/− MLFs were treated with LY294002 for 2 h and cellular protein was collected for western blot analysis of Akt substrate phosphorylation. Depicted blots are representative of three independent experiments.
