Figure 6
From: ASB2 is a novel E3 ligase of SMAD9 required for cardiogenesis
Accumulation, but not ablation of Smad9 attenuates cardiac differentiation. (a) Immunostaining of P19CL6 cells with anti MF-20 antibody after 12 days of induction of cardiac differentiation. The stable expression of Asb2 does not affect the cardiac induction while that of Smad9 suppress differentiation into cardiomyocytes. (b) MF-20 positive area. P19S shows siginificantly reduced MF-20 positive area. (c) The expression level of the common major cardiac marker Myh6 and TnnT2. P19S showed decreased expression of both markers. The data are shown as the mean ± s.d. Statistical analysis was performed using one-way ANOVA with Tukey’s multiple comparison tests. *p < 0.05; ns, not significant.
