Table 1 Candidates of SMAD9 interacting proteins and their E3 activity.

From: ASB2 is a novel E3 ligase of SMAD9 required for cardiogenesis

Entrez GeneID

Gene symbol

Gene name

E3 activity

60

ACTB

Actin beta

No

160

AP2A1

Adaptor related protein complex 2 alpha 1 subunit

No

1442

CSH1

Chorionic somatomammotropin hormone 1

No

1583

CYP11A1

Cytochrome P450 family 11 subfamily A member 1

No

1877

E4F1

E4F transcription factor 1

No

2313

FLI1

Fli-1 proto-oncogene, ETS transcription factor

No

2512

FTL

Ferritin

No

2896

GRN

Granulin

No

3646

EIF3E

Eukaryotic translation initiation factor 3 subunit E

No

4245

MGAT1

Mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase

No

5467

PPARD

Peroxisome proliferator-activated receptor delta

No

5660

PSAP

Prosaposin

No

5714

PSMD8

Proteasome 26S subunit, non-ATPase 8

No

5859

QARS

Glutaminyl-tRNA synthetase

No

6904

TBCD

Tubulin folding cofactor D

No

7266

DNAJC7

DnaJ (Hsp40) Homolog, Subfamily C, Member 7

No

8544

PIR

Pirin

No

8663

EIF3C

Eukaryotic translation initiation factor 3 subunit C

No

8665

EIF3F

Eukaryotic translation initiation factor 3 subunit F

No

9070

ASH2L

ash2 (absent, small, or homeotic)-like (Drosophila)

No

9093

DNAJA3

DnaJ (Hsp40) Homolog, Subfamily A, Member 3

Yes

11034

DSTN

Destrin

No

22943

DKK1

Dickkopf WNT signaling pathway inhibitor 1

No

23462

HEY1

Hes related family bHLH transcription factor with YRPW motif 1

No

23650

TRIM29

Tripartite motif containing 29

No

26508

HEYL

hes related family bHLH transcription factor with YRPW motif-like

No

29979

UBQLN1

Ubiquilin 1

No

51523

CXXC5

CXXC Finger Protein 5

No

51676

ASB2

Ankyrin repeat and SOCS box containing 2

Yes

55236

UBA6

Ubiquitin like modifier activating enzyme 6

No

55577

NAGK

N-Acetylglucosamine Kinase

No

55611

OTUB1

OTU Deubiquitinase, Ubiquitin Aldehyde Binding 1

No

56893

UBQLN4

Ubiquilin 4

No

57104

PNPLA2

Patatin like phospholipase domain containing 2

No

64129

TINAGL1

Tubulointerstitial nephritis antigen like 1

No

64333

ARHGAP9

Rho GTPase activating protein 9

No

64374

SIL1

SIL1 nucleotide exchange factor

No

64795

RMND5A

Required for meiotic nuclear division 5 homolog A

No

79048

SECISBP2

SECIS Binding Protein 2

No

79230

ZNF557

Zinc finger protein 557

No

80325

ABTB1

Ankyrin repeat and BTB (POZ) domain containing 1

No

81570

CLPB

ClpB homolog, mitochondrial AAA ATPase chaperonin

No

  1. The potent interacting proteins of SMAD9 were narrowed down using 3 individual databases of interactome. MINT and BioGRID are common database of protein interaction provided on the web. Rual et al. provided human interactome database in the previously published study (see reference19). The thorough literature search revealed that, among these candidates, only DNAJA3 and ASB2 have the E3 activity.