Figure 3
From: Nonspecific binding of common anti-CFTR antibodies in ciliated cells of human airway epithelium

Super-resolution microscopy of 596 immunofluorescence reveals ring-like pattern at the bases of cilia. (a) Representative image of the apical side of a non-CF HBE cell co-immunostained with 596 and centrin-2 antibody; left side: confocal image, right side: super-resolution STED image. (b–e) Representative STED images of apical membrane fluorescence in HBE cells obtained using 596, 528, 796 and 450 antibodies.