Figure 1

Schematic for the quail egg drug screening assay. The quail eggs are incubated for 72 h and then the embryos are transferred to six-well plates. The six-well plates are incubated for 96 h then any non-viable egg contents are removed. During the quail egg incubation, tumor cells are cultured. After the 96 h incubation the tumor cells are suspended in hydrogel, added to a 3D scaffold, and applied to the chorioallantoic membrane. A 9 mm ring is placed onto the 3D scaffold and then filled with P-10 osmotic beads soaked and equilibrated in the therapeutic compound. The quail is then incubated for 72 h. To analyze drug efficacy on tumor cells, luciferin is added to the scaffold and then imaged by a CCD camera to determine the total xenograft cell viability.