Figure 3
Effect of different pre-incubation modes with A. platensis (a), N. punctiforme (b), C. reinhardtii (c) and H. pluvialis (d) extract (concentrations: 2.1 mgde mL−1, 0.7 mgde mL−1, 1.8 mgde mL−1 and 7.1 mgde mL−1) on viral replication of CyHV-3. Either cells, virus suspension or both were pre-incubated with the ethanolic extracts before inoculation with CyHV-3 at 25 °C for 1 h. Infectious supernatant was replaced with extract (in medium) and cells were incubated at 25 °C for 72 h. The viability of the cells was approximately 100% for each experiment, only for the incubation with H. pluvialis the cell viability was slightly affected and reduced to 90%. Each bar of viral DNA copy numbers (shown in gray) and infectious viral titer (shown in black) represents the mean of three biological replicates ± SD. In addition, the vitality of CCB cells normalized to the growth control is shown. Initial viral DNA copy number and virus titer are shown by dashed lines, while the respective limit of quantification is represented by dotted lines. If the virus titers could no longer be detected using TCID50, this is marked with < LOD (limit of detection). *p ≤ 0.05 (compared to replication control, RC).
