Figure 3

MTD12813 promotes bacterial clearance, neutrophil recruitment and phagocytosis in Kp-1705 bacteremia. (a-d) BALB/c mice were challenged i.p. with Kp-1705 (3 - 5 x 10⁸ CFU) and treated with 1.25 mg/kg MTD12813 (M) or saline control (C) 1 h p.i. (a) Mice were euthanized 4 or 24 h p.i. and bacterial burden determined. Data are geometric means ± 95% CI with P values calculated using Student’s t-test. (b) At 2, 4, and 24 h p.i., peritoneal lavage fluid was collected and cytospin preparations stained with H & E. (c) Peritoneal neutrophils were collected and counted 24 h p.i. from sham (S) and Kp-1705 (Kp) infected mice treated 1 h p.i. with saline (C) or 1.25 mg/kg MTD12813 (M). (d) Blood neutrophils from infected animals treated 1 h p.i. were quantified at t = 0, 2, 4 and 24 h, data shown as means ± 95%CI (n = 5 for sham n = 9-12 for trx groups), P values determined by Student’s t-test with Welch’s correction. (e) Naïve BALB/c mice (2M/2F per time point) received 1.25 mg/kg MTD12813 by a single i.p. injection, and blood neutrophil numbers were determined by CBC analysis. Graph depicts means ± SD, P values determined by Student’s t-test comparing each time point to t = 0.