Figure 1
MPA inhibits wound closure by endometrial epithelial cells. (A) Wound closure of endometrial epithelial cells grown in transwell inserts at 24 h post-scratch following pre-treatment for 48 h with 100 nM progesterone. Each symbol represents a single individual (n = 10). (B) Representative images of endometrial epithelial cells grown in 96-well plates scratched using the IncuCyte WoundMaker in the absence (control) or presence of MPA (100 nM) at 0, 12, 24, and 48 h at × 10 magnification using the IncuCyte Zoom. Time of scratch is 0 h. The solid white line represents the epithelial wound front. (C) Relative wound density of endometrial epithelial cells grown in 96-well plates was measured at the indicated time points for 24 h post-scratch in the presence (100 nM) or absence of MPA. Data shown is from another representative patient than (B). Each symbol represents the mean + /− SEM from a single timepoint. Each timepoint is the average of triplicate or quadruplicate wells. (D) Wound closure of endometrial epithelial cells grown in transwell inserts at 24 h post-scratch following pre-treatment with 100 nM MPA, NET, and LNG for 48 h prior to scratch. MPA, NET, LNG, and Progesterone were maintained in cell culture media at 100 nM following scratch. Each symbol represents a single individual (n = 22).
