Table 1 Independent variables and levels in the Box–Behnken design.
From: Optimisation of enzymatic saccharification of wheat straw pre-treated with sodium hydroxide
Independent variables | Symbols | Range and levels | ||
|---|---|---|---|---|
− 1 | 0 | 1 | ||
Solid content (%, w/v) | X1 | 2.0 | 5.0 | 8.0 |
Enzyme loading (FPU/g substrate) | X2 | 10 | 22.5 | 35 |
Temperature (°C) | X3 | 40 | 45 | 50 |
pH | X4 | 4.0 | 5.0 | 6.0 |
Hydrolysis time (h) | X5 | 12 | 54 | 96 |