Table 1 Independent variables and levels in the Box–Behnken design.

From: Optimisation of enzymatic saccharification of wheat straw pre-treated with sodium hydroxide

Independent variables

Symbols

Range and levels

 − 1

0

1

Solid content (%, w/v)

X1

2.0

5.0

8.0

Enzyme loading (FPU/g substrate)

X2

10

22.5

35

Temperature (°C)

X3

40

45

50

pH

X4

4.0

5.0

6.0

Hydrolysis time (h)

X5

12

54

96