Figure 6
Protein level of Ki-67, P62, and LC3 measured by ELISA and PCR array of 86 genes involved in autophagy machinery. (A) Ki-67 protein level (as a proliferation indicator), significantly decreased followed by 43 and 48 °C, which shows the reverse effect of temperature elevation on the cell’s proliferation ability (P < 0.01). (B) LC3 protein level (as autophagy indicator) showed different behavior of cells in response to temperature elevation (P < 0.0001). (C) Interestingly, our results showed that the P62 protein level does not change significantly in response to temperature increase. (D) Phylogenic tree of 86 genes related to autophagy. Our results showed that 54 genes from those changed in response to the PTT. One-Way ANOVA and Tukey post hoc analysis (n = 3). **P < 0.01; ***P < 0.001; ****P < 0.0001.
