Table 7 GAD, GABA-T and SSADH genes expression and enzymes activity in petals of rose flowers treated with PSKα at 0 and 150 nM during vase life at 20 °C for 12 days.
PSKα treatment (nM) | Vase life (days) | Genes relative expression | Enzyme’s activity (μmol kg−1 min−1) | ||||
|---|---|---|---|---|---|---|---|
GAD | GABA-T | SSADH | GAD | GABA-T | SSADH | ||
0 | 0 | 4.26 ± 0.28 | 3.57 ± 34 | 2.96 ± 0.31 | 54.67 ± 4.42 | 27.48 ± 1.46 | 17.20 ± 1.21 |
4 | 10.04 ± 0.61 b | 3.98 ± 0.25 d | 4.34 ± 0.14 c | 59.26 ± 3.60 b | 30.78 ± 1.54 c | 21.46 ± 0.65 d | |
8 | 5.33 ± 0.62 cd | 5.32 ± 0.35 c | 7.50 ± 0.27 b | 42.35 ± 1.32 c | 32.29 ± 1.65 c | 32.37 ± 1.61 c | |
12 | 2.64 ± 0.18 d | 6.61 ± 0.35 b | 8.55 ± 0.26 b | 29.38 ± 0.97 d | 51.73 ± 0.94 ab | 42.69 ± 1.66 b | |
150 | 4 | 16.19 ± 1.74 a | 4.28 ± 0.16 d | 5.28 ± 0.21 c | 67.69 ± 1.92 a | 35.37 ± 1.61 c | 26.39 ± 0.78 d |
8 | 7.74 ± 0.75 bc | 7.41 ± 0.34 b | 10.77 ± 0.88 a | 62.66 ± 1.51 ab | 46.98 ± 1.74 b | 46.69 ± 2.24 b | |
12 | 3.52 ± 0.83 d | 10.39 ± 0.44 a | 11.83 ± 0.64 a | 48.89 ± 3.40 c | 57.62 ± 3.25 a | 54.07 ± 2.30 a | |
Significant | df | ||||||
|---|---|---|---|---|---|---|---|
Treatment | 1 | ** | ** | ** | ** | ** | ** |
Time | 2 | ** | ** | ** | ** | ** | ** |
T × T | 2 | * | ** | * | * | * | * |
CV | – | 21.01 | 9.00 | 10.36 | 7.88 | 7.84 | 7.75 |
LSD (P = 0.05) | 1.01 | 1.48 | 7.25 | 5.92 | 5.14 | ||