Figure 5
From: A high-throughput pipeline for scalable kit-free RNA extraction
SARS-CoV-2 detection on hypothetical patient samples. Saliva and throat swab samples were collected from 24 volunteers and spiked 8 each with 1 × 105 copies/mL heat inactivated 2019 novel coronavirus (nCoV; blue diamond), VLPs (red diamond), or water (green diamond). Total RNA was extracted using (A) Opentron-2 and (B) Eppendorf epMotion. Biological samples were analysed by SYBR one-step RT-qPCR targeting SARS-CoV-2 N gene. Artefact amplification was observed in water-spiked saliva and throat swab samples (green diamond).
